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KMID : 0360220070480111548
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Journal of the Korean Ophthalmological Society
2007 Volume.48 No. 11 p.1548 ~ p.1553
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Effect of Nitric Oxide on the Trabecular Meshwork Cell-mediated Contraction of Collagen Gels
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Kim Jae-Woo
Chang Woo-Seok
Lee Su-Yoon
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Abstract
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Purpose: To investigate the effect of nitric oxide (NO) on the contraction of cultured human trabecular meshwork cells (HTMCs).
Methods: After embedding them into collagen gels, primarily cultured HTMCs were exposed to NO donors, such as sodium nitroprusside (SNP) or S-Nitroso-N-acetylpenicillamine (SNAP), for 1 week at various concentrations, and the contraction of the collagen gels was measured. Cellular survival and NO production were measured with MTT assay and Griess assay, respectively.
Results: Though SNP and SNAP did not significantly affect cellular survival, they markedly enhanced NO production. Both sodium nitroprusside and SNAP inhibited the contraction of collagen gels by about 10% in dose and time-dependent manners (p<0.05).
Conclusions: NO donors inhibited the contraction of collagen gels in vitro. Thus, NO donors may relax trabecular meshwork and enhance trabecular outflow.
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KEYWORD
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Collagen gel, Contraction, Nitric oxide, Trabecular meshwork
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